Potential of rod, sphere and semi-cube shaped gold nanoparticles to induce cytotoxicity and genotoxicity in human blood lymphocytes in vitro
نویسندگان
چکیده
Aim: Gold nanoparticles (GNPs) are intended to be used in nanomedicine. Due to nanotechnology innovation GNPs of variable sizes and in different shapes including rods, spheres, cubes, etc., can easily be produced. The aim of the present studies was to evaluate the cyto-and genotoxicity inducible by different shaped GNPs on normal human peripheral blood lymphocytes. Methods: Four different shapes of GNPs including big rod GNPs (BR-GNPs, 50 nm), small rod GNPs (SR-GNPs, 30 nm), sphere GNPs (S-GNPs, 15 nm) and semi-cube GNPs (SC-GNPs, 15 nm) were studied. Cultured human blood lymphocytes were treated with different concentrations of these GNPs for 24 h in vitro. Cytotoxicity was evaluated based on the mitotic index (MI), while genotoxicity was studied by an interphase-fluorescence in situ hybridization (I-FISH) assay. The following genes were studied in I-FISH: TP53, CASP3, CASP7, CASP9, TNF and CRP. The induction of cell death was investigated using the apoptotic-necrotic assay. Results: The lowest concentration of BR-GNPs neither had an effect mitotic activity nor enhanced gain or loss of examined gene signals in a significant manner with I-FSH. Other concentrations of BR-GNPs, SR-GNPs, S-GNPs and SC-GNPs with all concentrations inhibited the mitotic activity of the cells and reduced the cell proliferation highly significantly. The different types of GNPs initiated the duplication of CASP3, CASP7 and CASP9 besides TP53 genes; however they reduced the signals of TNF and CRP genes highly significantly as supported by I-FISH results. The apoptotic-necrotic assay demonstrated that small rod, sphere and semi-cube GNPs enhanced the cell death rate through the apoptotic pathway. Conclusion: GNPs at high concentration can reduce the cell proliferation and induce DNA damage. Low concentration of rod-shaped GNPs at 50 nm was safe on human lymphocytes. Further research studies are required to optimize the concentration, shape and size of GNPs before using them in nanomedicine.
منابع مشابه
Investigation of cytotoxicity properties of zinc oxide nanoparticles in spherical and rod shaped on leukemia cells
In this study, we reported a method to associate doxorubicin drug on folic acid functionalized SiO2/ZnO nanoparticles (NPs) in rod and spherical shapes. The clinical usage of the anticancer drug, doxorubicin (DOX), is limited by severe side effects and cell resistance. Targeted drug delivery by binding DOX to nanoparticles could overcome these limitations. The surface functionalization of the Z...
متن کاملAntioxidant effects of gold nanoparticles on Schistosoma mansoni induced granuloma, in vitro
Objective(s): Schistosomiasis and hepatitis C virus [HCV] co-infection is common among the Egyptian population. Co-infected patients have higher rate of chronic hepatitis, cirrhosis and hepatocellular carcinoma. The aim of the present study was to investigate the potential role of gold nanoparticles on granuloma in vitro. Materials and Methods: In the current study, granulocytes were isolated f...
متن کاملDiverse Cytotoxic Capability of Silver Nanoparticles against the Normal and Cancerous Lymphocytes
Despite the toxic effects on numerous organs, silver nanoparticles can be used to cease cell cycle and promotes apoptosis in cancerous cells. Therefore, by choosing the right dosage nanoparticles can be helpful for cancer treatment, whilst do not harm normal tissues. In the current study, it has been tried to measure silver nanoparticles toxicity against HPB-ALL leukemia cell line and normal hu...
متن کاملDiverse Cytotoxic Capability of Silver Nanoparticles against the Normal and Cancerous Lymphocytes
Despite the toxic effects on numerous organs, silver nanoparticles can be used to cease cell cycle and promotes apoptosis in cancerous cells. Therefore, by choosing the right dosage nanoparticles can be helpful for cancer treatment, whilst do not harm normal tissues. In the current study, it has been tried to measure silver nanoparticles toxicity against HPB-ALL leukemia cell line and normal hu...
متن کاملبررسی و مقایسه مکانیسم سمیت ژنتیکی نانوذرات مس و سولفات مس
Background and purpose: Copper is a trace element that is used in various industries. Copper nano-particles are being used in different skin care products, lubricants, oils, polymers, metal covers, plastics, inks, etc. Previous studies have shown that both copper sulfate and copper nano-particles can induce cellular genotoxicity and DNA damage. So, this study aimed at evaluating and comparing g...
متن کامل